Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQD----------------------------------------------------------------------LDAAKMLVMSHLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKK--LTLEEAQSIANDLN--VTPEQVLEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDNR-YDPARLVENEDWEEQSTSALHDAMNQLDDRSRNILQRRWLDD--DKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS 2A6H Chain:F ((74-413)) ------------KISTSDPVRQYLHEIGQVPLLTLEEEVELARKV-EEGMEAIKKLSEITGLDPDLIREVVRAKILGSARVRHIPGLKETLDPKTVEEIDQKLKSLPKEHKRYLHIAREGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKFEYKRRFKFSTYATWWIRQAINRAIADQARTIRIPVHMVE--TINKLSRTARQLQQELGREPTYEEIAEAMGPGWDAKRVEETLKIA-QEPVSLETPIGDEKD---SFYGDFIPDEHLPSPVDAATQSL----LSEELEKALSKLSEREAMVLKLRKGLIDG-----EEVGAFFGVTRERIRQIENKALRKLKYHES--

General information: TITO was launched using: RESULT: Template: 2A6H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 862 23825 27.64 92.34 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain F : 0.69 3D Compatibility (PKB) : 27.64 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_2A6H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A6H-query.scw PDB file : Tito_Scwrl_2A6H.pdb: