Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence M----KRILIVTGQSGSGKSSALQVLEDLGYYCIDNLPLALLPEIVAKLDHENNLEQLALGVDVRSTRAD----MQEFDHVFEQLQKH-GTVDVIYLTTQDQDLIARFSASRRPHPLANRFKSLLQCIHEEKQLLIPIQFCATVHIDTTDKSVHDLKHILLSKLGQSDNLIVILQSFGYKHGIPLDADYVFDVRHLPNPHWDLELRRFSGLDEPVKQFLEASP--QANEMFEDILHFLKKWLPAFAEGHRHYMTISIGCTGGQHRSVYMVDRLKQALEAEWSVQVLHREMKHWS--- 1A4U Chain:A ((1-254)) MDLTNKNVIFVAALGGIGLDTSRELVKR-------NLKNFVILDRVENPTALAELKAINPKVNITFHTYDVTVPVAESKKLLKKIFDQLKTVDILINGAGILD-DHQIERTIAINFTGLVNTTTAILDFWDKRKGGPGGIIANICSVTGFNAIHQVPVYSASKAAVVSFTNSLAKLAPIT-----GVTAYSINPGITRTPLVHTFNSWLDVEPRVAELLLSHPTQTSEQCGQNFVKAIEA---------------------NKNGAIWKLDL---------GTLEAIEWTKHWDSHI

General information: TITO was launched using: RESULT: Template: 1A4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1320 26227 19.87 109.28 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 19.87 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_1A4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A4U-query.scw PDB file : Tito_Scwrl_1A4U.pdb: