Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLSVMQSLQSLQPRISVAPMMDWTTKDYRFFARLFNPNVVLYTEMVTTGAILFG------DAKR----------HLD-YNAQEHPIVLQLGGSNPQELATCTKMAEDWGYDEVNLNVGCPSDRVQNNKIGACLMAEPDLVAECIHSMQKAVNIPVTVKHRIGIDDMQSYEEMLHFVDTVAATGCTHFVVHARIAILKGLSPKENREVPPLRYEDVYRLKQERPHLTIEINGG----IKTFAETQAHLQ--HVDGVMIGREAYHNPYLLAELGQLWNLEAPDRFDIMEQMLPYIEQRLAEGAPLSIITRHILGLFQNLPGARKWRQALSGGNAKTLTDVENAIHNMQAAIIRTEEYLKEHQF 4WFS Chain:A ((7-308)) --------------HLILAPMVRVGTLPMRLLALDY-GADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVE-NDVAGIDVNMG-------------ALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSL----EDTLSLVKRIERTGIAAIAVHGRKRE-------E-RPQHPVSCEVIKAIADTL-SIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGL------RPLEEVMQKYIRYAVQYDN---HYTNTKYCLCQMLREQLE-SPQGRLLHAAQ--SSREICEAF-------------------

General information: TITO was launched using: RESULT: Template: 4WFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1484 -39383 -26.54 -148.06 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -26.54 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_4WFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WFS-query.scw PDB file : Tito_Scwrl_4WFS.pdb: