Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MLNS--------EFQPNWLYPPSEVIKNFIQLNNIDTSAFDVSTKKIIDDLVNQNTKID-------LYTSEVLK------EAIGGTTQF--------WLNIQNQFTINSLRIQNSSVDNNFSEYRNLVN-----ELKKLHWTPE-SKYDYLDQINLKSFFGIS--EFSLLTKKY-----IVQHNLRVKFKNIGQYNSSELNVATLIRKAELEAKKQPILSPWNKLLFIEKLQEIKKLSKVKGFKNFRNELLSICNQCGVAFIVLDTLNKSPIRGISKFLDSNIGLIIVTTKYNKDDVFWQTFFHEAGHLVLHSKEMVFSDQGDLNKEASHNLEEAQADEFMLTHILHPY----TIEEVTNMFNTRIMYKSKTESWRNICQIA----RQVNISPSLLTGILKFQG----IIPYSYFTNGHEKLYD------------- 3J9O Chain:B ((79-505)) VIDKLIDLQVNSIISNDEFRALEQEWLKVQEVCQEDY---DNVEVSILDVKKEELQYDFERNLYDISSSDFFKKVYVSEFDQYGGEPYGAILGLYNFENTTNDIIWLTGMGMVAKNSHAPFIASIDKSFFGVKDLSEITHIKSFEALLEHPRYKEWNDFRNLDVAAYIGLTVGDF-MLRQPYNPENNPVQYKLMEGFNEFVDYDKNE---SYLWGPASIHLVKNMMRS-YDKTRWFQYIRGVESGGYVKNLVACVYDNKGILETKSPLNVLFADYMELSLA--------NIGLIPFVSEKGTSNACFFSV--NSAKKVEEFVDGFDSANSRLIANLSYTMCISRISHYIKCVIRDKIGSIVDVESIQKILSDWISEFVTTVYQPTPLEMARYPFRNVSIEVKTIPGKPGWYSCKINVIPHIQFEGMNTTMTIDTRLEPELFGTNN

General information: TITO was launched using: RESULT: Template: 3J9O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1553 24927 16.05 70.81 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 16.05 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.139

(partial model without unconserved sides chains): PDB file : Tito_3J9O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J9O-query.scw PDB file : Tito_Scwrl_3J9O.pdb: