Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLNFRMIPKDQYPSDVKILRVVSINVNGLRSSVTKGLLEWLEQSDADVVCMQESRITHEQWTEKF-RPEGWHTHLFPAERAGYAGTAIYSRLPFVSIKDGLGFELADSQGRFISAEFDLGLSHPVHIASLYLPSGSSGEEAQARKDLFLGEYAKILKQWRDENKSIIICGDYNIVHKRIDIKNWSGNQKSSGCLPHERAWLDHIYDELGYVDTFRVVRTEAELYSWWSNRGQARAKNVGWRIDYHACSPDWKARTVNAWVYKDQWFSDHAPVIIDYKIQE 5J8N Chain:A ((2-257)) -----------------EHYNLISWNVNGLRAAVKKGFLDLLLEHRFDIVCVQETKVSQDKLPREVKNIQGYYNYFVSAEQNGYSGVGTFSKNKPIKLEKGMGIEVFDREGRFL--RT---DYEDFVLLNIYFPNGKMSQERLGYKMAFYDAFLDYANALKSEGKKLVICGDVNTAHKEIDLARPKQNEMISGFLPEERAWMDKF-LAAGYLDSFRMFNPEGGNYSWWSYRTGARSRNVGWRLDYVFVSENLRENVKSASIYPEIMGSDHCPVGLELEF--

General information: TITO was launched using: RESULT: Template: 5J8N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1421 -65470 -46.07 -256.75 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -46.07 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_5J8N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J8N-query.scw PDB file : Tito_Scwrl_5J8N.pdb: