Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNVSMTQRISEVVRNTNETKIRVRLNLDGTGQGTLNTGVPFLDHMIDQIKQHGLFDIDIHCDGDLEIDDHHTVEDCGITLGQAFAQALGDKKGLRRYGHFYAPLDEALSRVVVDLSGRPGLFMDIPFTRARIGTFDVDLFSEFFQGFVNHALMTLHIDNLKGKNSHHQIESVFKALARALRMACEIDPRAENTIASTKGSL 5DNX Chain:B ((3-176)) --------------RTTKETDIIVEIG----KKGEIKTNDLILDHMLTAFAFYLGKDMRITATYDLR---HHLWEDIGITLGEALRENLPEK--FTRFGNAIMPMDDALVLVSVDISNRPYANVDVNIKDAEEG-FAVSLLKEFVWGLARGLRATIHIKQLSGENAHHIVEAAFKGLGMALRVATKESERVE----STKGVL

General information: TITO was launched using: RESULT: Template: 5DNX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 963 -132001 -137.07 -758.63 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -137.07 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_5DNX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DNX-query.scw PDB file : Tito_Scwrl_5DNX.pdb: