Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMLLEFTKMHGLGNDFMVVDLISQRAYLDTATIQRLADRHFGVGFDQLLIVEPPDVPEADFKYRIFNADGSEVEQCGNGVRCFARFVHERHLTNKTNITVQTKAGIVKPELGQNGWVRVNMGYPKFLPNEIPF-VADEPEALYTLELANDQNISIDVVNMGNPHAVTIVPDVLTADVAGIGPQVESHKRFPERINAGFMQVIDDKHVRLRVFERCVGETLACGTGACAAAVSGMRRGLLANSVEVELAGGKLQIEWQEGDVVWMTGPTTHVYDGRLDLRYFQG
5HA4 Chain:B ((3-282))MLLEFTKMHGLGNDFMVVDLISQRAYLDTATIQRLADRHFGVGFDQLLIVEPPDVPEADFKYRIFNADGSEVEQCGNGVRCFARFVHERHLTNKTNITVQTKAGIVKPELGQNGWVRVNMGYPKFLPNEIPFVAEEPEAL-YTLELANDQNISIDVVNMGNPHAVTIVPDVLTADVAGIGPQVESHKRFPERVNAGFMQVIDDKHVRLRVFERGVGETLACGTGACAAAVSGMRRGLLANSVEVELAGGKLQIEWQEGDVVWMTGPTTHVYDGRLDLRYFQ-


General information:
TITO was launched using:
RESULT:

Template: 5HA4.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1672 -94015 -56.23 -336.97
target 2D structure prediction score : 0.61
Monomeric hydrophicity matching model chain B : 0.98

3D Compatibility (PKB) : -56.23
2D Compatibility (Sec. Struct. Predict.) : 0.61
1D Compatibility (Hydrophobicity) : 0.98
QMean score : 0.574

(partial model without unconserved sides chains):
PDB file : Tito_5HA4.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-5HA4-query.scw
PDB file : Tito_Scwrl_5HA4.pdb: