Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAILKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEITPFLDP-EKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKTLELLWGLENECLPTSELPSHFMSHIARLE-VDPDNRIVMHCHASHLLAMSFTHELDERSFSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSGKDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVHTKAGV 2V9N Chain:B ((3-272)) -------NITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAPYHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAFNLGIVKVDSDGAGYHILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVGILPWMVPGTDAIGQATAQEMQKHSLVLWPFHGVFGSGPTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASAL---------

General information: TITO was launched using: RESULT: Template: 2V9N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1476 -42626 -28.88 -159.05 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -28.88 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_2V9N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V9N-query.scw PDB file : Tito_Scwrl_2V9N.pdb: