Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMGSKKQEEIMKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFVWHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHISEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYH-NIPWLITENGMGREYEEQYMDEKGIVQDDYRIEFIGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK 5GNX Chain:A ((8-456)) --------TARSYRFPEGFLWGAATAAYQIEGSSMADGAGESIWDRFSHTPGNM-KDGDTGDVACDHYNRWREDIELMKRLNLQAYRFSVSWSRVIPQGRG-AINPKGLAFYDRLVDGLLEAGIEPLATLYHWDLPAALDDR-GGWLNPDIADWFADYGQVLFEKFKGRVKTWGTINQPWVIVDGGYLHGALAPGHRSAYEAVIAGHNVLRAHGAAVRRFREVGE-GQIGIVLNIEPKYPASDKPEDEAARRRAEAQMNRWFLDPLMGRGYPEELTDVYG-AAW-REFPKEDFELIA-EPTDWMGLNWYTRAVPENAPDAWPTR--SR-------PVRQTQHAHTETGWEVYPPALTDTLVWLSEQTGGKLPLMVTENGSAWYDPP-H-AIDGRIHDPMRVHYLQTHIKALHDAIGKGVDLRGYMAWSLLDNLEWSLGYSKRFGIVHVNFAT-QERTIKDSGLLYAEVIKTHGDVL------

General information: TITO was launched using: RESULT: Template: 5GNX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2690 -151412 -56.29 -337.97 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -56.29 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_5GNX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GNX-query.scw PDB file : Tito_Scwrl_5GNX.pdb: