Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGH-HYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKG-MKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN-------QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQV--TDTLVSMFTE------------- 5L3X Chain:B ((1-405)) GEITSVSTACQQLEVFSRVLRTSLATILDGGE----ENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAMLSKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQDHKHKYIHILAYAASVVETW-----KKNKRVSINKDELKSTSKAVETVHNLCCNENASELVAELSTLYQCIRFPVVAMGVLKWVDWTVSEPRYFQL------------QPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSALDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIADPVTEFIAHCKSNFIMVN

General information: TITO was launched using: RESULT: Template: 5L3X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1431 28489 19.91 78.48 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 19.91 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_5L3X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L3X-query.scw PDB file : Tito_Scwrl_5L3X.pdb: