Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISEFDRNNPVLKDQLSDLLRLTWPEEY-----GDSSAEEVE----EMMNPERIAVAAVD-QDELVGFIGAIPQYG---ITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNAN---GWDKPDIWMAKTIIPRPNSQ 2J8M Chain:B ((4-171)) -SIRDAG---VADLPGILAIYNDAVGNTTAIWNETPVDLANRQAWFDARARQGYPILVASDAAGEVLGYASYGDWRPFEGFRGTVEHSVYVRDDQRGKGLGVQLLQALIERARAQGLHVMVAAIESG-------------------------NAASIGLHRRLGFEISGQMPQVGQKFGRWLDLTFMQLNLDPTRSA-

General information: TITO was launched using: RESULT: Template: 2J8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 608 -8342 -13.72 -54.88 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -13.72 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_2J8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J8M-query.scw PDB file : Tito_Scwrl_2J8M.pdb: