Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQRDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFIQNYTSIAVTGSHGKTSTTGLLSHVLS--GVR------PT------SYLIGDG-T---------------------------G--------------HG-DPQAEFFSFEACE---YRRHFLAYSPDYAIMTNIDFDHPDYYT-SIDDVFTAFQTMAGQVK---KAIFAYGDDAYLRKLKA-NV--PIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVIAVAY-F-EK-LDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQKYPDKEIIAVFQPHTFTRTIALMDEFAEAL-DLADKVYLCDIFGSAREEQGNVKIEDLGAKIKK----GG-EVI-KENNVSPLL-D-------------YHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL 2VOS Chain:A ((63-482)) -------------------------------------------------------------------------------------------------------SYPSIHIAGTNGKTSVARMVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQYVATYREIEPLVALIDQQSQASPAMSKFEVLTAMAFAAFADAP-VDVAVVEVGMGGRWDA-TNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPSPDTVAVIGRQVPKVMEVLLAESVRADASVAREDSEF-AVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFFGAQLDGDAVRAGFAAVT-SPGRLERMRS-APTVFIDAAHNPAGASALAQTLAHEFDFRFLVGVLSVLG-DK---DVDGILAALEPVFDSVVVTHNGSPRALDV-----EALALAAGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAG--RTGIVITGSVV---TAGAARTLF--------

General information: TITO was launched using: RESULT: Template: 2VOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1745 -15368 -8.81 -48.94 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -8.81 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_2VOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VOS-query.scw PDB file : Tito_Scwrl_2VOS.pdb: