Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKGKARAYTNIALIKYWGKKN-EELILPMNNSLSLTLDA-FYTETEVIFSDSYMVDEFYLDGTLQD-EKATKKVSQFLDLFRKEAG--LSLKASVISQNFVPTAAGLASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDL--SSYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQLKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIPCYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE 5GMD Chain:A ((2-323)) LKSVTVSAPSNIAVVKYWGKRGDERLNLPLNNSLSITLDDQLSVITKVTLNDKN---IVIVNDRILSEDEMKEYAGRVLDTFKKIVGKEFHVKVESKSK--FPINAGLASSAAGIAALAFSLNELLELNLKSEELSKIARLGSGSACRSMFGGFVVWNKGEREDGEDSYCYQIFRHDYWSELVDIIPILSEKEKKISSRKGMIRSAETSELMECRLKYIEKTFNEVIEAIRNRDEKKFYYLMMRHSNSMHAVILDSWPSFFYLNDTSIRIMEWIHDYGKAG--YTFDA--GPNPHIFTTERNIGDILEFLKSL-EIKRIIVSKVGDGPKVLS

General information: TITO was launched using: RESULT: Template: 5GMD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1784 18896 10.59 59.99 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 10.59 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_5GMD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GMD-query.scw PDB file : Tito_Scwrl_5GMD.pdb: