Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREYKGLKIAVAGTGYVGLSIATLLAQ-SHTVTAVDIIPEKVELINNKKSPIQDDYIEMYLAK--KELDLMATVDGVSAYKDADFVVIAAPTNYDSKKNFFDTSAVEAVIKLVMEV-N---PEAIMVIKSTIPVGYTASIREKT----------GSKNIIFSPEFLRESKALYDNLYPSRIIVGTDMEDERLVEAAQTFAKLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQQIINGVCRDPRIG--THYNNPSFGYGGYCLPKDTKQLLANYE--DVPENLIEAIVESNRTRKNFIADRVLELAGAYEANSHWDASKEKKVVIGVYRLTMKSNSDNFRQSSIQGVMKRVKAKGTTVVIYEPSLKDGEIFF--------------GSKVVNDLDQFKAMCDSIIANRYDTCLDDIK-------EKVYTRDIFQRD 1MV8 Chain:C ((1-425)) ------MRISIFGLGYVGAVCAGCLSARGHEVIGVDVSSTKIDLINQGKSPIVEPGLEALLQQGRQTGRLSGTTDFKKAVLDSDVSFICVGTPSKKN-GDLDLGYIETVCREIGFAIREKSERHTVVVRSTVLPGTVNNVVIPLIEDCSGKKAGVDFGVGTNPEFLRESTAIKDYDFPPMTVIGELDKQ-----TGDLLEEIYRELD----APIIRKTVEVAEMIKYTCNVWHAAKVTFANEIGNIAKAVGVDGREVMDVICQDHKLNLSRYYMRPGFAFGGSCLPKDVRALTYRASQLDVEHPMLGSLMRSNSNQVQKAFDLITS---------------HDTRKVGLLGLSFKAGTDDLRESPLVELAEMLIGKGYELRIFDRNVEYA-RVHGANKEYIESKIPHVSSLLVSDLDEVVASSDVLVLGNGDELFVDLVNKTPSGKKLVDLVGFMPH-

General information: TITO was launched using: RESULT: Template: 1MV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1748 -7244 -4.14 -18.91 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -4.14 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_1MV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MV8-query.scw PDB file : Tito_Scwrl_1MV8.pdb: