Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNYLDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV--QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSVITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQDLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLTLFGYWCYRKFKRQKQRKKNNR 4G1J Chain:B ((44-246)) ----------------------------------------------HANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSA-----GVVEYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVLTAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGEDHVTLLTCTPYMINSHRLLVRGKRIPYVE--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4G1J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1047 -22335 -21.33 -113.95 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -21.33 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_4G1J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G1J-query.scw PDB file : Tito_Scwrl_4G1J.pdb: