Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIPQGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVHPSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFDHITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGEYDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISV---PLSKLNVGLKCGGSDG--LSGITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFKQYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLSLLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDFNAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL------ 4ZOX Chain:A ((53-431)) -----------------------MSNNSLTYFDKHT-DSVFAIGHHPNLPLVCTGGGDN-------LAHLWT---------SHSQPPKFAGTLT-GYGESVISCSFTSEG-GFLVTADMSGKVLVHMGQKGGAQWKLASQMQEVEEIVWLKTHPTIARTFAFGATDGSVWCYQINEQDGSLEQLMSGFVHQQDCSMGEFINTDKGENT---LELVTCSLDST----IVAWNCFTGQQLFKITQAEIKGLEAPWISLSLAPETLTKGNSGVVACGSNNGLLAVINCNN-----------GGAILHLSTVIEL---------KPEQDELDASIESISWSSKFSLMAIGLV----------CGEILLYDTSAWRVRHKFVLEDSVTKLMFD--------------NDDLFASC------------INGK-------VYQFNARTGQEKFVCVGH----NMG--------------VLDFILLHPVANTGTEQK-RKVITAGDEGVSLVFEVPN

General information: TITO was launched using: RESULT: Template: 4ZOX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2276 -36443 -16.01 -99.03 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -16.01 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.139

(partial model without unconserved sides chains): PDB file : Tito_4ZOX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZOX-query.scw PDB file : Tito_Scwrl_4ZOX.pdb: