Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLRSAMHASDVDDAGRALQSTQRSI-VSISRLTNEMSEVRAMSEGSFGVVKCYRHDFDKLEYAVKQTKRPICGESNLQQQLQEIYALSSFPHRHIVRYFDGWVEDRAVFVRLE-----KLDDCMASLPPPVSESVLTAMLHQTSTALYELHSHDVVHMDVKPENILRRQLDADTFIFKLCDFGLARPLNGKDSVTGEHFLGLNDDDGDRRYMSPELLKNLHDVVGPPADMYALGKSCEAMMT-----ATEDPSNTSARHLE-----------SYSPAFIALIESMLCEDPARRPSAFQVVQATLPERLLSDGGLLELQRRIDAIRCEISDLDASDNDVMSSPRT--------------------------- 1KOB Chain:A ((23-374)) ------INDYDKFYEDIWKKYVPQPVEVKQGSVYDYYDILEELGSGAFGVVHRCVEKATGRVFVAKFINTP--YPLDKYTVKNEISIMNQLHHPKLINLHDAFEDKYEMVLILEFLSGGELFDRIAAEDYKMSEAEVINYMRQACEGLKHMHEHSIVHLDIKPENIMCETKKASS--VKIIDFGLATKLNPDEIVKVTT--------ATAEFAAPEIVDR--EPVGFYTDMWAIGVLGYVLLSGLSPFAGEDDLETLQNVKRCDWEFDEDAFSSVSPEAKDFIKNLLQKEPRKRLTVHDALEHPWLKGDHSNLTSRIPSSRYNKIRQKIKEKYADWPAPQPAIGRIANFSSLRKHRPQEYQIYDSYFDRKEA

General information: TITO was launched using: RESULT: Template: 1KOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1464 29860 20.40 98.55 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 20.40 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_1KOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KOB-query.scw PDB file : Tito_Scwrl_1KOB.pdb: