Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence EDGCSASILKYDANGGQETYYGSALPANGSI--AFQPTEFSTEVVISVDAN-----CNPTNTDDVKKSVPK-GWGLHVYKKVTAIAGES 1MWY Chain:A ((2-74)) ----SGTRYSWKVSG-----MDCAACAR-KVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVESALQKAGYSLRDEQ-----AAE-

General information: TITO was launched using: RESULT: Template: 1MWY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 266 4805 18.06 73.92 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 18.06 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_1MWY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MWY-query.scw PDB file : Tito_Scwrl_1MWY.pdb: