Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGESQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLYSQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNEVKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK 5IFZ Chain:B ((22-149)) MKVAVAGDSAGEGLAKVLADHLKD-----RFEVSEIS-------------NLSDRVASAVLDGTYDRAILVCGTGIGVCIAANKVPGIRAALTHDTYSAERAALSNNAQIITMGAR----VIGAEVAKTIADAFLAQTF--------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 660 -94991 -143.93 -811.88 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -143.93 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_5IFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IFZ-query.scw PDB file : Tito_Scwrl_5IFZ.pdb: