Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVMQLLPEL----NSGGVERGTLEIARALVAQGHQSLVVSNGGRLVSQL----------------------EAEGSTHLTLPIHK--K---------SLS----SL-------WQ-IRPLRQLIEEHQPDIVHVRSRVPAWLTHFALRKIPANKHPHLISTVHGFYSVN-----------------------------RYSAIMTQAEKVIAVSDSVVKYITDHY---K-------NCPPQDIVRIYRGIDPAAFPHNYQP-------------SAQWFNQVFNDFPE--LENKFLLCLPGRITRLKGHESLIELMQKLGEQYPQLHAVVVGGADVKKQTYLSELQNTIQSKGLADKI-TFVGHR-SDIREWLAFSDIVLSLSNQAETFGRTALEALSVGTPVIGWNRGGVAEILSHV---------YPQGLVEAENEKALVERVKHHIE---QPQTV------APVTMFSLKDMCDQTLELYQSVLK 3D1J Chain:A ((1-475)) MQVLHVSSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAFPDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFAYTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAAR---GRPAKSVFTVHNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYAREITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAENKRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQ--GGQLALLGAGDP---VLQEGFLAAAAEYP--GQVGVQIGYHEAFSHRIMGGADVILVPSR-FEPCGLTQLYGLKYGTLPLVRRTGGLADTVSDSSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRFVQRQAMAMDFSWQVAAKSYRELYYR---

General information: TITO was launched using: RESULT: Template: 3D1J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1808 -4469 -2.47 -12.70 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -2.47 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_3D1J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D1J-query.scw PDB file : Tito_Scwrl_3D1J.pdb: