Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIV-KIENVRVLDPIQKTDSVQTVYLQDGKLVAPV-D----QVEQTIDGQGKWLMPTMVDLCARLREPGQ--QQHGTLKSEGKAARANGILHVITPPDSKPIVQDNGALIHGLIEKAWHDGGIHMHIIGAQTHGLNGKQPANMAGLK-KGGCTAVS-NA---NAAFENDDVVVRTLEYAAGLGLTVVFYAEEPQLAKDGC---AHEGFIASRQGLPMIPAIAETVAIAKHLLMIEATGVKAHFGLLSCGASVELIRAAKAKGLPVTADVAMHQLHLTEQLID----GFNSLAHVRPPLRSAQDKELLRQGLKQGVIDAICTHHEPLSSSAKM---APFAETQPGITAFDTYVALGIQ-LINEGLFEPLEWVTKVTSAPAQVANM-T-AR---WQAEAGWVLVDPELSWTVSKDTILSQGKNTPLLGQKLTGKVLQTFAV 5E5C Chain:C ((2-432)) ---SLLIRGATVVTHEE--SYRADVLCANGLIQAIGENLETPSGCDVLDGGGQYLMPGGIDPHTHMQLPFMGTVASEDFFSGTAAGLAGGTTSIIDFVIPNPRQSLL-EAFHTWRGWA-QKSAADYGFHVAITWWS-DEVAREMGELVAQHGVNSF-HFMAYKNAIMAADDTLVASFERCLELGAVPTVHAENGELVFHLQQKLLAQGLTGPEAHPLSRPPQVEGEAASRAIRIAETLGTPLYLVHISSREALDEIAYARAKGQPVYGEVLAGHLLLDDSVYRHPDWATAAGYVMSPPFRPVEHQEALWRGLQSGNLHTTATDHCCFCAEQKAMGRDDFSKIPNGTAGIEDRMALLWDAGVNSGRLSMHEFVALTSTNTAKIFNLFPRKGAIRVGADADLVLWDPQGSRTLSAATHHQRVDFNIFEGRTVRGIPSHTIS-

General information: TITO was launched using: RESULT: Template: 5E5C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2392 4552 1.90 11.35 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : 1.90 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_5E5C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E5C-query.scw PDB file : Tito_Scwrl_5E5C.pdb: