Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQEVYCVMIEKSINPHDYQHIHSPASVMPKNYHAKHRIDLHQHERGQLIYATSGIMELS-----TEEEL---WLIPPRFGVWIPAYTPHQMTTKTDVHLNTLYIDTYRWSKPFPDKPCGVTVSGLLHELLIRAASFPIEY-PLHSVEWKTMDLLVEELKWTKGISLMTPEPYMDKRLQDLCNILIINPADNHSLEEWSSQVGATTRTLSRLFKQEMNTTFTLWRQQLRIVHAIPSLMAGK-PISDISQELGYSSQSTFTVMFKKIMGKTPTEYIAWANGNPTTFD 3GBG Chain:A ((4-273)) KKSFQTNVYRMSKFDTYIFN-----NLYINDY--------------KMFWIDSGIAKLIDKNCLVSYEINSSSIILLKKNSIQRFSLTSLSDENINVSVITISDSFIRSLKSYILGNKDLLLWNCEHNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYNSIFITDDLDAMEKISCLVKSDITRNWRWADICGELRTNRMILKKELE-SRGVKFRELINSIRISYSISLMKTGEFKIKQIAYQSGFASVSYFSTVFKSTMNVAPSEYLFMLTGV-----

General information: TITO was launched using: RESULT: Template: 3GBG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1149 -24239 -21.10 -96.95 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -21.10 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.324

(partial model without unconserved sides chains): PDB file : Tito_3GBG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GBG-query.scw PDB file : Tito_Scwrl_3GBG.pdb: