Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHYTGIDQLINSFDQALRSLVPGATAAQRQNPAETVEAKLGVEDARHVAGLMRVNHSGEVCAQALYHGQALTAKLP---NVRREMQQAAIEEQDHLAWCEDRLKELNSHTSLLNPI-WYGLSYGMGALAGIAGDKYSLGFVAETERQVSLHLQDHLNQLPA-QDERSRKILEQMNEDELHHRHTALEAGGVELPYAVKITMTAISKLMTKTSYYL 5I4J Chain:D ((15-163)) ---------------------------------------LLDRSVTAPVTEGFNVVLASFQALYLQYQKHHFVVEGSEFYSLHEFFNESYNQVQDHIHEIGERLDGLGGVPVATFSKLAELTCFEQ-ESEGVYSSRQMVENDLAAEQAIIGVIRRQAAQAESLGDRGTRYLYEKILLKTEERAYHLSHF---------------------------

General information: TITO was launched using: RESULT: Template: 5I4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 573 -87 -0.15 -0.60 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain D : 0.57 3D Compatibility (PKB) : -0.15 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_5I4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I4J-query.scw PDB file : Tito_Scwrl_5I4J.pdb: