Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRCNVMKILIIDNYDSFTFNLYQLLGEILISLKSRKEISDFEIVVKRNNEISLDEIRKLEFDRIIISPGPGSPDDER-YFGISKQVI-LQLGKEIPILGVCLGMQGIAYCFGGKVIRAKLPMHGKISTINH-----------------------------SDSGVFSGIPQ-DVEVMRYHSLIVDSSTLPECFIVTARVNSAYSDSEEEIMGIKHQTYPIEGVQFHPESFASEAGKTLLQNFVHSKL 3UOW Chain:A ((7-255)) ----YDKILVLNFGSQYFHLIVKRLNNIK-----------IFSETKDYGVE-LKDIKDMNIKGVILSGGPYSVTEAGSPH-LKKEVFEYFLEKKIPIFGICYGMQEIAVQMNGEVKKSKTSEYGCTD-VNILRNDNINNITYCRNFSSAMDLYSNYKLMN--CCLFENIKSDITTVWMNHNDEVTK--IPENFYLVSSS------ENCLICSIYNKEYNIYGVQYHPEVYESLDGELMFYNFAY---

General information: TITO was launched using: RESULT: Template: 3UOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 848 -73757 -86.98 -400.85 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -86.98 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_3UOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UOW-query.scw PDB file : Tito_Scwrl_3UOW.pdb: