Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQTPEALTTEQFKQAIIDKGQYYH-IYHPFHVMMYEGKATQQQIQAWVANRYYYQINIPLKDAAIMANCPDQRVRQEWIQRM-IDQDGEYPDGGGREAWLRLAEAVGLSREQVISEELVLPGVRFAVDAY-VNFARRASWREAASSSLTELFAPQIHQSRLESWPQHYPWIDDKGYEYFRSRLSQARRDVEHGLTITLDSF-TTHEQQQRMLEILQFKLDILWSILDALTLAYVHNEAPYHSVTQERVWHKGLFK 1RCW Chain:A ((7-219)) -----------NFLDQLDLIIQNKHMLEHTFYVKWSKGELTKEQLQAYAKDYYLHIKAFPKYLSAIHSRCDDLEARKLLLDNLMDEENGY---PNHIDLWKQFVFALGVTPEELEAHEP-SEAAKAKVATFMRWCTGDSLAAGVAALYSYESQIPRIAREKIRGLTEYFGFSNPEDYAYFTE---HEEADVRHAREEKALIEMLLKDDADKVLEASQEVTQSLYGFLDSFL-------------------------

General information: TITO was launched using: RESULT: Template: 1RCW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 921 -6443 -7.00 -30.83 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -7.00 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_1RCW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RCW-query.scw PDB file : Tito_Scwrl_1RCW.pdb: