Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKSPGFTIVELTITLVILVIMSVIAIPLYHQFMASVELKNTLRILTIHIQKAKYDAIIRHKNVVLCPSSDQLVCNTNWDNRIISFVDNNRNLQHDLNEELLASID-LNH-HYGSMKLQR----FGKKQ-NSIIFQGNSGLPIESNGSFIYCSYDQLKKREFGLSRI 5IFG Chain:B ((3-130)) ---------------------------AIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPE---------NPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTSMVSRVLSGKRKLTLEHA---------KKLATRFG----

General information: TITO was launched using: RESULT: Template: 5IFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 197 -14485 -73.53 -130.50 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -73.53 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.259

(partial model without unconserved sides chains): PDB file : Tito_5IFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IFG-query.scw PDB file : Tito_Scwrl_5IFG.pdb: