Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKMLIRKAKKDDTDTIVAIIAPYVDQVIANDEGRSRFKSEAIQTIFDR------EDIHYFVGEIDQQIVGIVAYMEPSHLMH-FFIKKTHLKLGLGRQLWDFIEEKIKNENIDIEKITVNSSFYAQDIYEKFGFIVSGDAAEKWGIRFIPMTK-YYALASEK 2FIW Chain:A ((1-160)) GHMVMSTPALRPYLPEDAAVTAAIFVASIEQLTADDYSEEQQEAWASAADDEAKFAARLSGQLTLIATLQGVPVGFASLKGPDHI-DMLYVHPDYVGRDVGTTLIDALEKLAGARGAL--ILTVDASDNAAEFFAKRGYVAKQRNTVSINGEWLANTTMTKSL----

General information: TITO was launched using: RESULT: Template: 2FIW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 596 -22085 -37.05 -150.23 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -37.05 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_2FIW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FIW-query.scw PDB file : Tito_Scwrl_2FIW.pdb: