Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSIFDLHIQVQPQHIDALGHVNNVMYVQWMQDVAAAHVETLGVGVTKYLELKHAMVAVEHHVQYRKAAFEGEEIVLRTWLDDINALY-SFRQYAFFRPS---DQAILFVGNTKWACIEIAS--GRPKRMSPTFTQAYKPLDSSINPLDFTVSYA 4I4J Chain:G ((8-149)) NPDYFELRHTVGFEETNLVGNVYYVNYLRWQGRCRELFLKERAPSVLAEVQEDLKLFTLKVDCEFFAEITAFDELSIRMRLSE-LRQTQLEFTFDYIKLGDDGGETLVARGRQRIACMRGPNTATVPTLIPEALAEALAPYSD------------

General information: TITO was launched using: RESULT: Template: 4I4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 566 -45062 -79.61 -331.34 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain G : 0.66 3D Compatibility (PKB) : -79.61 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_4I4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I4J-query.scw PDB file : Tito_Scwrl_4I4J.pdb: