Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRMLHTMLRVGNLEQSLKFYTEVLGMKLLRKRDYEEGRFTLAFVGYGDEEN---------------NTMLELTHNW-----DTSSYDLGN----GYGHIAIGVEDAYKACEEIKARGGKVVREA--GPMKGGVTVIAFVEDPDGYKVELIQQDANARNN 3W0T Chain:B ((35-184)) --LQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKG----LAFIQDPDGYWIEILNPNKMA---

General information: TITO was launched using: RESULT: Template: 3W0T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 397 -5604 -14.11 -45.19 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -14.11 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.673

(partial model without unconserved sides chains): PDB file : Tito_3W0T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W0T-query.scw PDB file : Tito_Scwrl_3W0T.pdb: