Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVKILAVDMDGTFLNSKKQYNKARFLKQYEQLKQNNIRFVVASGNQLAKLVTYFPEINHEIAFIAENGAHVVDAGQELAFAHLSKEQFIEILNAIDPV-YTSKMVICGKQSAYVHSSMNAEDYAKVARYFE-KLTVIDDFYAL-DDLVCKITFTAQENESFTIFEHFQKQSFVKDKVLVPVSSGFNFIDLILPDQHKAHGLKLLLQKWQVQPDQVVAIGDNNNDIQMIKAAGYGFAVEA----LKSVTPYTTANNEQEGALQVIDLVLQHQPPFA 3DAO Chain:B ((20-283)) -MIKLIATDIDGTLVKDGSLLIDPEYMSVIDRLIDKGIIFVVCSGRQFSSEFKLFAPIKHKLLYITDGGTVVRTPKEILKTYPMDEDIWKGMCRMVRDELPACDYFAATPDFCFAEDGGS-PIFHLLRDSYGFEMREVDDITRLDRNDIIKFTVFHPDK-CEELCTPVFIP--AWNKKAHLAAAGKEWVDCNAKGVSKWTALSYLIDRFDLLPDEVCCFGDNLNDIEMLQNAGISYAVSNARQEVIAAAKHTCAPYWENGVLSVLKSFL-------

General information: TITO was launched using: RESULT: Template: 3DAO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1381 -87823 -63.59 -341.72 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -63.59 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_3DAO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DAO-query.scw PDB file : Tito_Scwrl_3DAO.pdb: