Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWNPNSIIFCFYFIFSILALWGIIEAWVHQSRTETIHPFKAFVHLLAFYLSYLLIPLWFFNLYAGWIGYYSIHETIFIFLLSGILIYARFIEPHMVRVDVHQYRLNPDRHFVKPVKVALIADLHIGLFSG-------HERQLKIIVKKLNEQQPDLVVVAGDWTYEPEDK---LVQELSVLK------------------------DIKAPVYSVPGNHDEQYPGPPIQQLLKDALYYNDVM-DI----EGKI-----VEF--D--EFRLIGIGDLWAG-------KTDMRSMPDL--PQDKPWLILSHNPDTVDM-------VPKLPNRPLMLSGHTHGGQVELPLVTNYIMKKVSILGHKRGFYSHEHADVFVTVGTGMVGIPLRFRVPPTIDIIELA 4FBW Chain:A ((10-254)) --------------------------------------------------------------------------------------------------------------NENTIRILISSDPHVGYGEKDPVRGNDSFVSFNEILEIARERDVDMILLGGDIFHDNKPSRKALYQALRSLRLNCLGDKPCELELLSNINYLDPNINVAIPVFSIHGNHDD-----RYS--ALDILQVTGLVNYFGRVP---NIVVSPILLQKGFTKLALYGISNVRDERLYHSFRENKVKFLRPDLYRDEWFNLLTVHQNHSAHTPTSYLPESFIQDFYDFVLWGHEHECLIDG-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 756 -11356 -15.02 -70.53 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -15.02 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_4FBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FBW-query.scw PDB file : Tito_Scwrl_4FBW.pdb: