Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATAVNCNICFIGGGNMAQALIGGLISRG-LPPTRITV---SDPVEQIRQLLQEKEVHVTQDNTAAIQNADVVVLAVKPQVLANVLRPLKGLLS-DKLVISIIAGAEIKTISNLIDSERIVRVMPNTPALVQTGAHGIYATDVVGASDRELTSQILAATGLTIWVNSEAQIDAVTAVSGSGPAYFFYLMESMIRAGKNLGLDEKVATALTLQTALGAAQMAITSSNTPSELRKNVTSPNGTTQAALEVFDRAQISQNIQSALAVAQKRSQELAQELSDSAK 5BSF Chain:C ((11-277)) ---ADSYTLGFIGAGKMAESIAKGAVRSGVLSPSRIKTAIHSNPAR--RTAFESIGITVLSSNDDVVRDSNVVVFSVKPQLLKDVVLKLKPLLTKDKLLVSVAAGIKMKDLQEWAGHERFIRVMPNTAATVGEAASVMSLGGAATEEDANLISQLFGSIG-KIWKADDKYFDAITGLSGSGPAYIYLAIEALADGGVAAGLPRDLALSLASQTVLGAASMATQSGKHPGQLKDDVTSPGGTTIAGVHELEKAGFRGILMNAVVAAAKRSQELS--------

General information: TITO was launched using: RESULT: Template: 5BSF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1112 -14296 -12.86 -54.56 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -12.86 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_5BSF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BSF-query.scw PDB file : Tito_Scwrl_5BSF.pdb: