Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----TQKCIITIRKADPALGQDTLYDLVSLAPNSDLTFGKIPASMTEVTFG-VDEKCQARLVRGDLSRPYEYKGMQNPLYGLDGPDFVKKLQAWRRKPE-NSINFSLW--------- 1UFX Chain:A ((1-103)) GSSGSSGTLVRVKKSAATLG-------IAIEGGAN--TRQPLPRIVTIQRGGSAHNC-GQLKVGHVIL--EVNGL--TLRGKEHREAARIIAEAFKTKDRDYIDFLVTEFNSGPSSG

General information: TITO was launched using: RESULT: Template: 1UFX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 326 22241 68.22 252.73 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 68.22 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_1UFX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UFX-query.scw PDB file : Tito_Scwrl_1UFX.pdb: