Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------CFVAIVSDDPRGPVLASKRFEGNREVSFDGYDIV-------LELGWDCYAIK--KSGTIPHGYHINIASGVDEPRVGVCNVYNDRTEPRRRNLHTQPTSN-- 1UFF Chain:A ((1-93)) GSSGSSGYRALYPFEARNH----------DEMSFNSGDIIQVDEKTVGEPGW-LYGSFQGNFGWFPCNY-VEKMPSSENEKA---VSPKKALLPPTVSLSATSGPSSG

General information: TITO was launched using: RESULT: Template: 1UFF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 266 5227 19.65 68.77 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 19.65 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_1UFF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UFF-query.scw PDB file : Tito_Scwrl_1UFF.pdb: