Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEIDRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGVEQIISTGTCGVLAD--IEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLEDRGIPYEEVMTWTTDGFYRETAEKVAYRK-EEGCAVVEMECSALAAVAQLRGVLWGELLFTADSLADLD--QYDSRDWGSEAF-NKALELSLASVHHL 3MB8 Chain:A ((9-246)) -------------------VQPHIRLRKEDVEPVVIIVGDPARTEEVANMCEKKQE---LAYNREYRSFRVVYDSQPITVISHGIGCPGTSIAIEELAYLGAKVIIRAGTCGSLKPKTLKQGDVCVTYAAVNETGLISNILPEGFPCVATPHVYQALMDAAKELGIEAASGIGVTQDYFYQNGILPSKLEMYSKCCDVIDMEMSGVLGLCQARGIATCGILAVDGSPLQWDEGDYDATGVKATTGKENMVKITLKACANL

General information: TITO was launched using: RESULT: Template: 3MB8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1373 -89389 -65.10 -385.30 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -65.10 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_3MB8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MB8-query.scw PDB file : Tito_Scwrl_3MB8.pdb: