Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTEMGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLAELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPP-----VDYKEEDYYQREDDKPETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK 4QBH Chain:A ((1-216)) MNLVLMGLPGAGKGTQAERIVEAYGIPHISTGDMFRAAMKEGTPLGLQAKQYMDRGDLVPDEVTIGIVRERLGKDDC-ERGFLLDGFPRTVAQAEALEEILEEIGRPIDYVIHIEVRQDVLMERLTGRRICRNCGATYHLIFHPPAKPGVCDKCGGELYQRADDNEETVAKRLEVNMKQMKPLLDFYEQKGYLRNVNGQQDMQDVFADVRELLGGLK

General information: TITO was launched using: RESULT: Template: 4QBH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 985 -26644 -27.05 -126.27 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -27.05 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_4QBH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QBH-query.scw PDB file : Tito_Scwrl_4QBH.pdb: