Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGLAANMIGVQKRVIIFNLG-LVP-VVMFNPVLLSF-EGSYEAEEGCLSLVGVR-STKRYETIRLAYRDSKWQEQTITLTGFPAQICQHELDHLEGRII 5VCP Chain:C ((39-158)) -------------------------LVKDMFETM--HDANGAGLAAPQIGVNLQVVIFGFGPPVPETVLINPTITPVSQDMEEGWEGCLSVPGLRGAVSRFSMIKYHGFDQYGKPIDRVAEGFHARVVQHECDHLIGKL-

General information: TITO was launched using: RESULT: Template: 5VCP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 500 -43747 -87.49 -405.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : -87.49 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_5VCP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VCP-query.scw PDB file : Tito_Scwrl_5VCP.pdb: