Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKK--HVEILEELFPGYENTWRSSQEPA-RKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEER-QVWDAKYAEYLAELDKEKPVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL 3W2Y Chain:D ((7-260)) LKIISWNVNGL---------RAVHRKGFLKWFMEEKPDILCLQEIKAAPEQLPRKLRHVE-------GY----RSFFTPAERKGYSGVA-MYTK--VPPSSLREGFGVERFDTEGRIQIADFDDFLLYNIYFPNGKMSEERLKYKLEFYDAFLEDVNRERDSGRNVIICGDFNTAHREIDLARPKENSNVSGFLPVERAWIDKFIENGYVDTFRMFNSD-PGQYTSWSYRTRARE-RNVGWRLDYFFVNEEFKGKVKRSWILSDVMGSDHCPIGLEIEL

General information: TITO was launched using: RESULT: Template: 3W2Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1353 24255 17.93 97.02 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : 17.93 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_3W2Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W2Y-query.scw PDB file : Tito_Scwrl_3W2Y.pdb: