Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIQLYLLKLELFWFELHQHYHFSEPQKILNELIAAYSAAYSEKQRAYHTVQHLYECLSLIETVQSE------LNDPYAVALALWFHDVVYEPQAL-----------------DNELKSAELFEQLMAQ-DL------QL----DTMQKIKRWILATQKHAPT-----------------------DETDLQFLLDIDLAILAATPERFMQYEQQIQQEYSWADPEVYSIKRKEVLMNFYQSEPLYQTAYFQKNFELNAKQNLKKILE 4LM4 Chain:A ((65-253)) --------------------------CRFIMSVKKN----YR--RVPYHNWKHAVTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFW-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LM4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 470 -28557 -60.76 -216.34 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -60.76 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.605

(partial model without unconserved sides chains): PDB file : Tito_4LM4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LM4-query.scw PDB file : Tito_Scwrl_4LM4.pdb: