Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKK--LTLEEAQSIANDLN--VTPEQVLEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDNR-YDPARLVENEDWEEQSTSALHDAMNQLDDRSRNILQRRWLDD--DKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS 4XLN Chain:L ((103-337)) ------------------------------------------------EGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKFEYKRRFKFSTYATWWIRQAINRAIADQARTIRIPVHMVE--TINKLSRTARQLQQELGREPSYEEIAEAMGPGWDAKRVEETLKIA-QEPVSLETPIGDEKD---SFYGDFIPDENLPSPVEAAAQSL----LSEELEKALSKLSEREAMVLKLRKGLIDGREHTLEEVGAYFGVTRERIRQIENKALRKLKYHES--

General information: TITO was launched using: RESULT: Template: 4XLN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 612 -29929 -48.90 -131.27 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain L : 0.69 3D Compatibility (PKB) : -48.90 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_4XLN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XLN-query.scw PDB file : Tito_Scwrl_4XLN.pdb: