Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNNSQNLIMLKHDFASHIEDLKCATKPRSEALGRHLWTCTI-------DADFYWLKFHLPNVHAQSEQDFLHELQFYEDITYKKANWLLPFKIIEGRTISQQTQFQGKVLV--LPDTECWFDDLDQ-----------KQNLKNINEKIYLALVKLAELHELGWIHGDIKKEHFRKFK-QELYLIDFEKTRLISSP-----DPITDATPRYMAPELFHGANKTVQSDLYALGIVLYEWLT-QTRLQANSYHEWAVLH--CQKLNVELPSS-FQIFLPLLSGLLQKQQQNRFSNVHEAINCLKALST 4ASE Chain:A ((57-345)) --------------SKWEFPRDRLKLGKPLGRGAFGQ-VIEADAFGIDKTATCRTVAVKMLKEGATHSEHRALMSELKILIHIGH-HLNVVNLLGACTK-------PGGPLMVIVEFCKFGNLSTYLRSKRNEFVPYYKDFLTLEHLICYSFQVAKGMEFLASRKCIHRDLAARNILLSEKNVVKICDFGLARDIYKDPDYVRKGDARLPLKWMAPETIFDRVYTIQSDVWSFGVLLWEIFSLGASPYPGVKIDEEFCRRLKEGTRMRAPDYTTPEMYQTMLDCWHGEPSQRPT-FSELVEHLGNL--

General information: TITO was launched using: RESULT: Template: 4ASE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1190 -8356 -7.02 -33.16 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -7.02 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_4ASE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ASE-query.scw PDB file : Tito_Scwrl_4ASE.pdb: