Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARRPKRYTEEDFDSLEGRAS------------------KTEQKKAVQRMAALGE--------------QLAQLSIKQIQKLPVDERLIDALLEVQNISSFEARRRQFQR--VGKLLRNEDE------TVILSYLT-------PQQGAK------KQAQLMRWVDRMIEQGDPAINEFSKIYNASERHTLRQHVLRINRDK---TQQVAEADL---EATKMKFINYVQ--QVALLSDQG----------------------- 5FLG Chain:A ((5-259)) ---------EETFYSVRMRASMNGSDGGKHISGGERLIPFHEMKHTVNALLEKGLSHSRGKPDFMQIQFEEVHESIKTIQPLPVHTNEVSCPEEGQKLARLLLEKEGVSRDVIEKAYEQIPEWSDVRGAVLFDIHTGKRMDQTKEKGVRVSRMDWPDANFEKWALHSHVPAHSRIKEALALASKVSRHPAVVAELCWSDDPDYITGYVAGKKMGYQRITAMKEYGTEEGCRVFFIDGSNDVNTYIHDLEKQPILIEWEEDHD

General information: TITO was launched using: RESULT: Template: 5FLG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 537 45929 85.53 271.77 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 85.53 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_5FLG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FLG-query.scw PDB file : Tito_Scwrl_5FLG.pdb: