Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILITGANTGIGFATAEQLVKQGQHVILACRNPQKAQEAQNKLRSLDQGQVDVASLDLNSLELTQKAAEEIADKYGSLDVLINNAGLFSKT--KQLTVDGFEQKFGVNYLGHFLLTQKLLPVLKQF-PQAHIIHLASIAHWVGSIKPNKFRAEGFYNPLFYYGQSKLANLLFSNALAEQLADSS--ITNNALHPGGVASDIYRDLPKPVYAAMKVGLVPTSVPAKLITEMATGDTWQNRNGEYVSAHMPDWKSSHAKNQQLARDLYQQSMNLVEKFL 5IG2 Chain:A ((15-205)) KVAVITGAGSGFGRAFAEKGASLGMKLVLADVDEGALARTVDTLRAA-GAEVIGVRTDVSNGAQVQALADAALEAFGKVHLLFNNAGVGAGGFLWESSANDWAWVFGVNVMGVAHGVRVFAPIMLGQNEAAHIVNTASVAGLLS------------PPSMGIYNASKHAVVSLTETLYHDLRNAGGEVGCSLLCPAFVPTGIAD--------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IG2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 997 -82585 -82.83 -444.01 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -82.83 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_5IG2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IG2-query.scw PDB file : Tito_Scwrl_5IG2.pdb: