Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEHPQGQKHCSFCGKTQSEVGKLIAGEDAYICNECVDVCLDLVQTSQQVEAGDWASKALPKPHEIRAALDQYVIGQDLAKKTLSVAVYNHYKRLKVGQS-GHVSKDVEIAKSNILLIGPTGSGKTLLAQTLARLLDVPFAMADATTLTEAGYVGEDVENIVQKLLQKADYDVEKA-QKGIIYIDEIDKITRKSENPSITRDVSGEGVQQALLKMIEGTVASIPPQGGRKHPQQEFIQIDTSNILFICGGAFAGLEKIVQQRQEKGGIGFTADVKNKDETKKLAELFRQVEPTDLVKFGLIPEFIGRLPVIATLEELDEEALMQILTEPKNALTRQYQYLFNMENVDLVFEDSALRAVAKRALE-----RNTGARGLRSILENVLLETMYDLPSRTDVGTVFINEAVINGEAEPVYKSERQPKEAVTHESVAKADLKVIDSKSA 1OFH Chain:B ((6-270)) --------------------------------------------------------------PREIVSELDQHIIGQADAKRAVAIALRNRWRRMQLQEPLRH-----EVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDSAGGAIDAVEQNGIVFIDEIDKICKKGEYSGA--DVSREGVQRDLLPLVEGSTVST------KHG-----MVKTDHILFIASGA-----------------------------------FQVARPSD-----LIPELQGRLPIRVELTALSAADFERILTEPHASLTEQYKALMATEGVNIAFTTDAVKKIAEAAFRVNEKTENIGARRLHTVMERLM-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1OFH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1191 28970 24.32 112.29 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : 24.32 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_1OFH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OFH-query.scw PDB file : Tito_Scwrl_1OFH.pdb: