Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLIRTRKKIVSSAIASSLSMIATTAMAQEAVSQLPTIHTKATQEESLKVDQSANS-KFVAPLKDTPKSVSILSQKLIKDTNSNTLLEALRYEPGITLGAGEGGTPFTDMPYIRGYSGQSSIYVDGVRNTTSQNRDMFAIEQVEVIKGSSSALGGGGSVGGSINLIPKVAHEGDVYQGSVQSGTDNYRHIQLDANKDFGNGIAGRVVIMGHENEKAG-QSNGAEYKRVGIAPSITFGLDTATRGTLSYYYLRSNDEPDAGIPFNNANPAKPPVGVTVTPGGGKPVDVKAGTYYGWKARDFDKRENHIGTFKLEHDFNENLTLSNIATYNKSKSDYVYTNADDSKGNIYRGTVARRALSRILDTDAYSDQLSLRGKFNTGSLKHSFNVGTEWSFQETDQGVHTFTNAAGETTSTILDSNIQNCTSAAAVANGWCTSLNNPG-NGAFTDKRGSITAQSTTRSHNVGIYALDSIEFNPQWLLNLGVRWDKFETEKKYNKDVDGRTPHKAGDKLESDTDYFSYQAGLVFKPTEDGSIYLSYATSANPVGVLAEGDTGSDSISDSGTASASANALKPEEARTFELGTKWDLFNNRANLTAAVFRTEKQNTRIQIDPNTTANAGKSKVDGFEISLNGKITDKWDVSTGYSYLDSEITEAAYNAVAQEGKPLPFVAKNSATLWSTYRVMPQLTLGAGVEYRDQVFVNTTAPKYLPTYTIYNAVAKYDVNKNVNLQLNINNISDKRYFTSAHAAHYAFEGNGRNAVLAINFKY 5FOK Chain:A ((28-719)) -------------------------------------------DETTYNVDRS-ASKKYTAPLLDTPKTVTVIPQQVIKDTGALTLADALRTTPGITFGA--------DRPFIRGFNAESDTFLDGMRDVASQTREVFNVEQIEVSKGPGSAYTGAGSTGGSLNLISKTAKQDNFTDAGFTWGSDQTRRTTLDVNRMIGDNAAFRLNLMKHDAH-VAGRDE-VSVSRWGVAPTVTFGFDTPTRATLSYYHLSTDDMPDYGLPLTNVNRSKA---N---P-SKPA-SVDRDNFYGLKDRDYRKSTTDSGTFRIEHDLNDNLTLSNSTRLVRTTLDYIVSNPDDSRGNVANGYVYRSAKSRNSTSKGWVNQTDLKANFETGFIKHTLVTGLEFSYEDVHNRPYAITSGGGAG---------NTCNARLL-ASGDCTSLNRPTPGDNWTGSITDGLAYTDTDTKTSAAYVFDTLKLSEQWELNLGLRYDDFDTKSSGYQTAGRNG-PAGYFKRENNSHFWNYQTGLVYKPAPNGSIYLAWSTSSNP------------------------------RNRNLELGTKWAFFDDALSLNAALFRTDKTNAR------LQVLDGEQRVQGVELGFNGKLTEKWKVFGGYTYLDSEIRKSTV--KSDEGNKMPQTAQNNFTLWTTYDLLQNFTIGGGTTYVDKQYGNTANSTYIPSYWRYDAMASYKVSKNVDLQLNVQNLTDKRYFDQVYSTHMAHVAPGRTALLGVNFHF

General information: TITO was launched using: RESULT: Template: 5FOK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4231 193373 45.70 297.04 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 45.70 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.223

(partial model without unconserved sides chains): PDB file : Tito_5FOK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FOK-query.scw PDB file : Tito_Scwrl_5FOK.pdb: