Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGSTITPWVVGNWKMNPMRANANQLIEEFKQLLQQNQIADENCHVGVAPVSIALTTVQAQLQDAARTVYTVAQDVSRVAGTGAYTGEVSAELLKDSQINFVLVGHSERRDIFGDNVEILKAKLQNALNAGMTVIYCVGESLEQREQGQAEQVVLQQICDIAPVVTAEQWQNQVVIAYEPIWAIGTGKTASPQDAQAMHAKIREGLCQLTPAGSNIAILYGGSVKAENAVELAACPDINGALVGGASLNAASFYQIVQAFAQSK 4G1K Chain:B ((30-270)) ---------VIGNWKMHG-RLSGNQAL--LTEVAQGAQAVHDNVAIGVCVPFPYLAQAQAQLQGG--RVSWGSQDVS-AHEQGAYTGEVAAGMVAEFGAAYAIVGHSERRAYHGESNETVAAKARRALAAGLTPIVCVGETLAEREAGTTEQVVGAQLDAVLAVLSPDE-AARIVVAYEPVWAIGTGKSATAEQAQQVHAFLRGRLA--AKGAGHVSLLYGGSVKADNAAELFGQPDIDGGLIGGASLKSGDFLAICRA-----

General information: TITO was launched using: RESULT: Template: 4G1K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1306 -159023 -121.76 -659.84 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -121.76 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_4G1K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G1K-query.scw PDB file : Tito_Scwrl_4G1K.pdb: