Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELNTPLTVGDFEIKNRLVMAPLTRARSGESRVPNDLMVEYYQQRANAGLILTEATVIGSKTVGYADTPGLWSQEQAQAWNKIIEAVHAQGSKIVVQLWHVGRISHPELL--DGDIPVAPSAIQPAGEVSLLRPK------RPYVTPRALSIEEIQEIVAQYKHSAELAKAAGFDGVELHAANGYLIDQFLQSNTNQRDDEYGGPVENRARLLLEVVDAFIEVWGAGRVGVHIAPRGDSHDMGDENPLATFGYVVEQLSQRNVAFIFSRE-YEAADSISP-----QLRKKFNGVWIANENLTPESAKRILREGQADAVSFGKAYIANPDLLQRLEQGLPLNELQPTTLYAKGAEGYTDYPALEAS 3GX9 Chain:A ((11-370)) ---LFTPLQLGSLSLPNRVIMAPLTRSRTPDS-VPGRLQQIYYGQRASAGLIISEATNISPTARGYVYTPGIWTDAQEAGWKGVVEAVHAKGGRIALQLWHVGRVSH-ELVQPDGQQPVAPSALKAEGAECFVEFEDGTAGLHPTSTPRALETDEIPGIVEDYRQAAQRAKRAGFDMVEVHAAAACLPNQFLATGTNRRTDQYGGSIENRARFPLEVVDAVAEVFGPERVGIRLTPFLELFGLTDDEPEAMAFYLAGELDRRGLAYLHFNEP----DITYPEGFREQMRQRFKGGLIYCGNYDAGRAQARLDDNTADAVAFGRPFIANPDLPERFRLGAALNEPDPSTFYGGAEVGYTDYPFLD--

General information: TITO was launched using: RESULT: Template: 3GX9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2059 -32516 -15.79 -95.35 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -15.79 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.654

(partial model without unconserved sides chains): PDB file : Tito_3GX9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GX9-query.scw PDB file : Tito_Scwrl_3GX9.pdb: