Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRPITAVIHRQALQNNLAVVRKAMPN-SKVFAVVKANAYGHGIERVYEAFK--AADGFALLDLEEAKRIRALGWTGPILLLEGV---FSPQDLFDCVQYQLSFTIHSEAQIEWVEQHPYPAQ---FDVFLKMNSGMSRLGF-KPQHYVQAWERLNNLANVAKITHMMHFSDAD---GDRFGQQGIDYQITAFEEIVKDLPGERSVSNSAAILRYQDQLKSDYVRSGIMLYGSSPDYPTHSIADWGLQPTMSLRSEIISVQHLEPNESVGYGSNFVAEQPMTIGIVACGYADGYQRISPTGTPVLVDSVRTRTVGRVSMDMLAVDLTGIESAKVGSEVVLWGQSSTGVVLPIDDVAVSSGTVGYELMCAVTARVQFINQV 3E6E Chain:B ((7-366)) --RPTRLHIDTQAITENVQKECQRLPEGTALFAVVKANGYGHGAVESAKAAKKGGATGFCVALLDEAIELREAGVQDPILILSVVDLAYVPL----LIQYDLSVTVATQEWLEAALQQLTPESNTPLRVHLKVDTGMGRIGFLTPEETKQAVRFVQSHKEFLWEGIFTHFSTADEIDTSYFEKQAGRFK--AVLAVLEELPRYVHVSNSATALWHPD-VPGNMIRYGVAMYGLNPS-GNKLAPSYALKPALRLTSELIHVKRLAAGEGIGYGETYVTEAEEWIGTVPIGYADGWLR-HLQGFTVLVNGKRCEIVGRVCMDQCMIRLA--EEVPVGPVVTLVGKDGNEEN-TLQMVAEKLETIHYEVACTFSQRI------

General information: TITO was launched using: RESULT: Template: 3E6E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1909 -33915 -17.77 -97.74 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -17.77 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_3E6E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E6E-query.scw PDB file : Tito_Scwrl_3E6E.pdb: