Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRQQIDVLVKQMNIDTAKGEVDARVQQIVVRLLGDLFQAIEDLDIQPSEVWKGLEYFTDAGQANELGLLAAGLGLEHYLDLRADEADAKAGMTGGTPRTIEGPLYVAGAPESVGFAHMDDGTETGKIDTLIIEGTVTDTDGNIIENAKVEVWHANSLGNYSFFDKSQ--SDFNLRRTIFTDADGKYVALTTMPVGYGCPPEGTTQALLNKLGRHGNRPSHVHYFVSAPGYRKLTTQFNIEGDEYLWDDFAFATRDGLVATAVDVTDPAEIQRRGLDHAFKHITFNIELVKEATAAPSTEVERRRASA 5UMH Chain:B ((14-317)) FDTKEVQDLLKAAAN-LNGDAGNARFRQIVHR-LSDLFKAIDDLDITPDEVWAGVNYLNKLGQDGEAALLAAGIGLEKYLDIRMDAADRAAGLDGGTPRTIEGPLYVAGAPVRDGVAKIDLDDDA-DAGPLVIRGTVTGTDGKPLAGALVECWHANSKGFYSHFDPTGAQTAFNLRGAVRTDANGKYEFRTLMPVGYGCPPQGATQQLLNGLGRHGNRPAHVHFFVSGDGHRKLTTQFNIEGDPLIWDDFAYATREELIPHVVDKTGGAAL-GM-KSDAYKEIEFDIVLTPLLDGRDNQVVHRPRASA

General information: TITO was launched using: RESULT: Template: 5UMH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1418 -32840 -23.16 -109.10 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -23.16 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_5UMH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UMH-query.scw PDB file : Tito_Scwrl_5UMH.pdb: