Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPQQIELVKSTVPVLREHGVTLTTYFYKRMLNNNPELKNVFNLDDQTSLRQPRALAAAVLAYAENIENPTVLAKAVERITTKHVSLDIQPDQYAIVGDNLLHSISEVLNVPFESELIEAWKQAYLQLADILIGVEKQKYEQLESLKGGWAGWRSFEITQID-PLESGKRFTLKATDHG----DVLTSPANAFISVKVQVPN--QQLEQPKAFKFTEAQEDNTYLFDVQPE------ENHTEFSVSNILLEHYRVGDQVQVSAPLTL 4G1V Chain:A ((2-259)) LAEKTRSIIKATVPVLEQQGTVITRTFYKNMLTEHTELLNIFNRTNQKVGAQPNALATTVLAAAKNIDDLSVLMDHVKQIGHKHRALQIKPEHYPIVGEYLLKAIKEVLGDAATPEIINAWGEAYQAIADIFITVEKKMYEE-----ALWPGWKPFEITAKEYVASDIVEFTVKPKFGSGIELESLPITPGQYITVNTHPIRQENQYDALRHYSLCSASTKNGLRFAVKMEAARENFPAGL---VSEYLHKDAKVGDEIKLSAPAG-

General information: TITO was launched using: RESULT: Template: 4G1V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 960 -44987 -46.86 -183.62 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -46.86 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4G1V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G1V-query.scw PDB file : Tito_Scwrl_4G1V.pdb: